FastQCFastQC Report
Thu 26 May 2016
SRR1511962_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511962_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1633386
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT58120.35582526114464064No Hit
CTGTAGGACGTGGAATATGGCAAGA55920.3423563076945682No Hit
GTCCTAAAGTGTGTATTTCTCATTT51210.31352050280827676No Hit
CTTTAGGACGTGAAATATGGCGAGG44130.27017496170531646No Hit
GTCCTACAGTGTGCATTTCTCATTT27290.16707624529658024No Hit
GTATCAACGCAGAGTACTTTTTTTT24730.15140328128195052No Hit
CTGAAGGACCTGGAATATGGCGAGA24720.15134205876626836No Hit
ATTTAGAAATGTCCACTGTAGGACG20270.12409803928771276No Hit
CTGTAGGACCTGGAATATGGCGAGA20020.12256747639565908No Hit
GTCCTTCAGTGTGCATTTCTCATTT19990.12238380884861264No Hit
TTTCTAAATTTTCCACCTTTTTCAG18460.11301676394924408No Hit
GAATATGGCAAGAAAACTGAAAATC17260.1056700620673864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAACG504.569456E-617.09734
CGGTAGG1351.8189894E-1213.3875211
CGTTAGA500.001485186613.3170611
TAGGACC6550.013.1963894
TCCAACG1450.013.10218218
CGATATT550.0030392412.1064181
ATAATAC1650.012.0890013
CCAACGA951.041084E-611.9988419
CGTTAGG802.829206E-511.8902331
GGCGAGG14000.011.6024519
GTCGAGG1001.9318304E-611.39889819
GGAACCG600.005882785711.0815835
TTAGGAC18250.010.9818283
GGTATCA7400.010.926161
TGTCGAG1408.361894E-910.85609218
GACGTGA16300.010.8387797
AGGACGT31600.010.7910185
GGACGTG30750.010.7804126
TCTAGGA2300.010.7522472
CAGTACT2300.010.7374354
Produced by FastQC (version 0.11.5)