FastQCFastQC Report
Thu 26 May 2016
SRR1511962_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511962_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1633386
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT62890.38502840112502495No Hit
CTGTAGGACGTGGAATATGGCAAGA58670.35919249950715876No Hit
GTCCTAAAGTGTGTATTTCTCATTT55570.3402135196456931No Hit
CTTTAGGACGTGAAATATGGCGAGG47780.29252117992930027No Hit
GTCCTACAGTGTGCATTTCTCATTT29680.18170842654461344No Hit
CTGAAGGACCTGGAATATGGCGAGA27170.16634157510839448No Hit
GTATCAACGCAGAGTACTTTTTTTT27080.16579057246725515No Hit
GTCCTTCAGTGTGCATTTCTCATTT22000.13468953450072427No Hit
ATTTAGAAATGTCCACTGTAGGACG20500.12550615714840216No Hit
CTGTAGGACCTGGAATATGGCGAGA20440.12513882205430926No Hit
TTTCTAAATTTTCCACCTTTTTCAG19200.117547230109723No Hit
GAATATGGCAAGAAAACTGAAAATC18100.11081275338468677No Hit
TATCAACGCAGAGTACTTTTTTTTT17050.1043843892380613No Hit
GGTATCAACGCAGAGTACTTTTTTT16940.10371094156555768No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7250.012.7640051
TAGGACC8300.012.3657124
AGGACGA907.4324926E-611.6151185
GTCCTAA14850.011.3067941
TATGTCG1104.997237E-711.22220616
GGCGAGG12750.010.80367819
GCCTAAC1252.1133565E-710.6849191
TGTAGGA29450.010.6488352
TCCAACG1252.2413042E-710.63943418
GGACCTG16800.010.6297356
TTGGACA1803.45608E-1110.5591974
TGCCGGT909.564396E-510.55014810
AGGACCT17100.010.5036245
GTATAAG2100.010.44868851
GCCGGTT1002.4121804E-510.44464611
CTGTAGG29300.010.386671
GTCCTAC28850.010.3502721
AAATGTC6350.010.3175467
ACCTTTT7550.010.31291115
GTAGGAC29600.010.1453913
Produced by FastQC (version 0.11.5)