FastQCFastQC Report
Thu 26 May 2016
SRR1511961_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511961_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1357202
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30140.22207453275194114No Hit
GGTATCAACGCAGAGTACTTTTTTT17520.1290891112745192No Hit
TATCAACGCAGAGTACTTTTTTTTT17030.1254787422948095No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC400.00528285814.24828710
TATTAGG852.664783E-713.4254862
GGTATCA6500.012.7283931
CCTACAC1104.9735354E-711.2259233
AAGGCGT600.00588026711.0820026
GTATCAA14500.010.9525731
GTCCTAA4200.010.868251
CTTACTC1106.0509046E-610.36239053
AGGACGT7650.010.305955
TAGGACG7850.010.2853874
GGACGTG7300.010.1494646
GCTTAGG750.002624840510.14371
TGTCGAG750.002649843510.13211518
AATCCCG951.6454692E-49.99879719
TAGCCTA1054.108592E-59.9511845
GTAGGAC7700.09.8689433
TCTAGAC1356.447808E-79.8506673
CTTGCAC1951.6370905E-109.7424183
GGACCTG5750.09.5814576
GTCCTAC8500.09.5097181
Produced by FastQC (version 0.11.5)