FastQCFastQC Report
Thu 26 May 2016
SRR1511961_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511961_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1357202
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT34430.2536836815742977No Hit
GGTATCAACGCAGAGTACTTTTTTT21620.15929832110474343No Hit
TATCAACGCAGAGTACTTTTTTTTT21310.15701421011758013No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAA759.1695983E-713.9991411
TTAGGAC4550.013.3683193
TAGGACC3050.012.4643134
CCGGTTT550.003081231412.08401712
GGTATCA7750.011.9464811
TATGTCG802.8803306E-511.86910516
TGGTATC2700.011.2640452
GGACGTG7300.011.1895236
TTTAGGA6550.011.1726872
TCCTATA1303.2430762E-810.9661982
ACGTGAA4600.010.9398158
TAGGACG7550.010.8257914
AGGACGT7500.010.7712435
GACGTGA4150.010.7532997
GTATCAA17500.010.6902531
GTCGAGG803.7770433E-410.68534519
TGTCGAG803.7770433E-410.68534518
CGTGAAA4650.010.61799059
ATGTCGA1002.4109308E-510.44481317
TAGGACA3750.010.3910824
Produced by FastQC (version 0.11.5)