FastQCFastQC Report
Thu 26 May 2016
SRR1511960_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511960_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1287396
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT39960.31039400464192834No Hit
CTGTAGGACGTGGAATATGGCAAGA37970.2949364453517022No Hit
GTCCTAAAGTGTGTATTTCTCATTT36630.2845278375884343No Hit
CTTTAGGACGTGAAATATGGCGAGG33510.2602928702590345No Hit
GTATCAACGCAGAGTACTTTTTTTT28230.2192798486246656No Hit
GTCCTACAGTGTGCATTTCTCATTT18980.1474293845871822No Hit
CTGAAGGACCTGGAATATGGCGAGA17580.1365547197598874No Hit
TATCAACGCAGAGTACTTTTTTTTT17120.1329816156023477No Hit
GGTATCAACGCAGAGTACTTTTTTT16720.1298745685088349No Hit
ATTTAGAAATGTCCACTGTAGGACG15540.12070877958297214No Hit
TTTCTAAATTTTCCACCTTTTTCAG13980.10859129591827223No Hit
GTCCTTCAGTGTGCATTTCTCATTT13740.10672706766216455No Hit
CTGTAGGACCTGGAATATGGCGAGA13340.10362002056865177No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGT601.4681154E-615.83148919
CGGTAGG801.2714008E-714.2649611
GGTATCA6450.013.4171731
CGGTTTC1001.0080839E-813.298449513
CCGGTTT1051.9954314E-812.66519112
TCCAACG1800.012.13747418
CCACGGC550.003071525812.0890313
TAGGACC5450.012.0256554
TGTCGAG1157.079689E-811.56386918
GGCGAGG8150.011.42198319
TGCCGGT1354.7311914E-911.25794810
CACCTTT5350.011.18561314
ATGTCGA1201.2762939E-711.08204217
TAATACC600.005881527411.0816124
GATATAC3800.011.0115491
GTCGAGG951.3613384E-510.99871719
TATGTCG951.3613384E-510.99871716
GCCGGTT1252.2416498E-710.63876111
CACTGTT4400.010.5783137
AGGACCT11200.010.4314155
Produced by FastQC (version 0.11.5)