FastQCFastQC Report
Thu 26 May 2016
SRR1511960_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511960_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1287396
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT41770.3244533927400738No Hit
GTCCTAAAGTGTGTATTTCTCATTT39420.3061994910656861No Hit
CTGTAGGACGTGGAATATGGCAAGA39210.30456829134159186No Hit
CTTTAGGACGTGAAATATGGCGAGG34800.2703130971356133No Hit
GTATCAACGCAGAGTACTTTTTTTT34670.26930330683022163No Hit
TATCAACGCAGAGTACTTTTTTTTT21430.16646004803494807No Hit
GGTATCAACGCAGAGTACTTTTTTT20880.16218785828136797No Hit
GTCCTACAGTGTGCATTTCTCATTT20120.15628446880369365No Hit
CTGAAGGACCTGGAATATGGCGAGA18610.14455536602568284No Hit
TTTCTAAATTTTCCACCTTTTTCAG15400.11962131310024265No Hit
ATTTAGAAATGTCCACTGTAGGACG14460.11231975243048758No Hit
GTCCTTCAGTGTGCATTTCTCATTT13560.1053288964700838No Hit
CTGTAGGACCTGGAATATGGCGAGA13460.1045521346967056No Hit
GAATATGGCAAGAAAACTGAAAATC13200.10253255408592228No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5650.013.4558684
GGTATCA6800.012.7696481
GGCGAGG9550.012.13503419
GTCGAGG550.003070021312.08982119
CCAACGA802.8681034E-511.87393119
TAATACT1850.011.8147974
ATAGGAC1459.640644E-1111.7970853
CCTACAC1459.640644E-1111.7970853
TCCAACG1459.822543E-1111.79158518
CCAACGT658.027835E-411.69125619
GTCCTAG1900.011.551031
TTAATAC1354.6984496E-911.263063
TGTAGGA20700.011.2477562
TAATACC1104.943413E-711.2310634
CCACCTT4400.011.22190213
GCCGGTT855.3490894E-511.17069111
CTAGGGC600.00585998711.0870743
TGGCGAA600.005880365611.08190518
AGGACCT12100.010.9954475
CTGTAGG20400.010.9454121
Produced by FastQC (version 0.11.5)