FastQCFastQC Report
Thu 26 May 2016
SRR1511959_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511959_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1403080
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT39100.27867263448983665No Hit
CTGTAGGACGTGGAATATGGCAAGA36280.25857399435527556No Hit
GTCCTAAAGTGTGTATTTCTCATTT35080.25002138153205805No Hit
CTTTAGGACGTGAAATATGGCGAGG32140.2290674801151752No Hit
GTATCAACGCAGAGTACTTTTTTTT25320.1804601305698891No Hit
CTGAAGGACCTGGAATATGGCGAGA17860.12729138751888702No Hit
GTCCTACAGTGTGCATTTCTCATTT17250.12294380933375146No Hit
TATCAACGCAGAGTACTTTTTTTTT15050.10726401915785273No Hit
GGTATCAACGCAGAGTACTTTTTTT14870.10598112723437011No Hit
ATTTAGAAATGTCCACTGTAGGACG14040.10006557003164465No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG350.002151146816.3070681
CGGTAGG602.5363752E-514.2686831
TCCAACG1700.013.96846618
CGGTTTC1153.783498E-1013.21538313
GGTATCA6500.012.8784011
TAGGACC6650.012.7123544
CCAACGA1155.333277E-912.38942119
CCGGTTT1103.8202415E-812.08907212
GCCCTAA802.827719E-511.8905691
ACGAAAT1552.5465852E-1111.64339212
GGCGAGG10050.011.62509919
CTATAGT2150.011.4866284
ACCTTTT5450.011.15426815
GGCGTGC600.005881825511.0816498
GCCGGTT1201.2772762E-711.08164911
GGCCTAA951.3402148E-511.0144211
AATGTCC5100.010.9885268
CCTATAC1303.2710886E-810.9598733
CGAAATC1658.0035534E-1110.93773213
GAACAGT4200.010.8554946
Produced by FastQC (version 0.11.5)