FastQCFastQC Report
Thu 26 May 2016
SRR1511959_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511959_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1403080
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT41730.2974171109273883No Hit
CTGTAGGACGTGGAATATGGCAAGA39190.27931408045157796No Hit
GTCCTAAAGTGTGTATTTCTCATTT37020.26384810559625965No Hit
CTTTAGGACGTGAAATATGGCGAGG35150.2505202839467457No Hit
GTATCAACGCAGAGTACTTTTTTTT28850.20561906662485388No Hit
GTCCTACAGTGTGCATTTCTCATTT18890.1346323801921487No Hit
CTGAAGGACCTGGAATATGGCGAGA18810.1340622060039342No Hit
GGTATCAACGCAGAGTACTTTTTTT17110.1219460045043761No Hit
TATCAACGCAGAGTACTTTTTTTTT16700.11902386178977678No Hit
GTCCTTCAGTGTGCATTTCTCATTT15270.10883199817544259No Hit
ATTTAGAAATGTCCACTGTAGGACG14920.10633748610200416No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6400.013.8094034
GGACGTA500.00150039613.2988526
GGTATCA6900.013.0007051
CCAACGA1001.44566E-712.34761219
AATCCCG3100.012.25569519
GGCGAGG8400.011.75963119
TGTAGGA19250.011.3545272
CTGTAGG19150.011.2124851
GACGTGG10150.011.1322077
AGGCCGT600.005878949511.08237656
GTATTAA1657.4578566E-1110.9889921
ACTGTTC6500.010.9559058
GGACGTT700.001492318910.8562066
GTAGGAC19000.010.8537063
GATATAC5950.010.7459781
TGTAGAA7000.010.7250912
TGGCGAG19600.010.61274418
ACCTTTT5550.010.60791415
AAATCCC3950.010.58023318
TCCAACG1803.45608E-1110.553514518
Produced by FastQC (version 0.11.5)