FastQCFastQC Report
Thu 26 May 2016
SRR1511958_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511958_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1356036
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT38640.2849481872162686No Hit
GTCCTAAAGTGTGTATTTCTCATTT36730.27086301543616836No Hit
CTGTAGGACGTGGAATATGGCAAGA35730.2634885799492049No Hit
CTTTAGGACGTGAAATATGGCGAGG32330.23841549929352907No Hit
GTATCAACGCAGAGTACTTTTTTTT28860.21282620815376582No Hit
GTCCTACAGTGTGCATTTCTCATTT20530.1513971605473601No Hit
GGTATCAACGCAGAGTACTTTTTTT17390.1282414331182948No Hit
TATCAACGCAGAGTACTTTTTTTTT17380.12816768876342516No Hit
CTGAAGGACCTGGAATATGGCGAGA16190.11939211053393863No Hit
CTGTAGGACCTGGAATATGGCGAGA15250.11246014117619298No Hit
ATTTAGAAATGTCCACTGTAGGACG14150.10434826214053314No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGG508.6300366E-515.2169831
GTGTTAC903.5845005E-813.7375541
GTATTAG1251.0004442E-1012.9344351
GGTATCA7600.012.7642451
TAGGACC6400.012.6154494
TCCAACG1001.4437865E-712.34875418
CCGTGCA550.003071267412.0892439
AGAACCG550.003071267412.0892435
GTCGAGG907.4668715E-611.6099419
ATGTCGA1001.9311356E-611.398849517
TGTCGAG1001.9311356E-611.398849518
ATTATAC1001.9319923E-611.3984293
GGCATAC600.005826370311.0957161
CCCTTAG951.3430017E-511.012291
AATGTCC4950.010.9378868
GGCGAGG9400.010.91379219
TAGGACA6200.010.8775334
TTAGGAC13000.010.7408273
AGGACCT13100.010.5863285
CTAAGGG909.525403E-510.5541013
Produced by FastQC (version 0.11.5)