FastQCFastQC Report
Thu 26 May 2016
SRR1511958_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511958_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1356036
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT40840.3011719452875882No Hit
GTCCTAAAGTGTGTATTTCTCATTT37370.27558265414782496No Hit
CTGTAGGACGTGGAATATGGCAAGA36500.26916689527416676No Hit
CTTTAGGACGTGAAATATGGCGAGG34290.2528693928479775No Hit
GTATCAACGCAGAGTACTTTTTTTT33410.24637988961944962No Hit
GTCCTACAGTGTGCATTTCTCATTT21520.15869785167945394No Hit
TATCAACGCAGAGTACTTTTTTTTT20380.15029099522431558No Hit
GGTATCAACGCAGAGTACTTTTTTT20020.14763619844900874No Hit
CTGAAGGACCTGGAATATGGCGAGA18890.13930308634874No Hit
CTGTAGGACCTGGAATATGGCGAGA15600.11504119359663018No Hit
GTCCTTCAGTGTGCATTTCTCATTT15080.11120648714340917No Hit
TTTCTAAATTTTCCACCTTTTTCAG14660.10810922423888451No Hit
ATTTAGAAATGTCCACTGTAGGACG14620.10781424681940598No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGTA350.002171330616.284236
CAGCGCA456.785165E-414.7709819
TGGACCG400.005272796414.252385
TAGGACC6450.013.552654
GGTATCA7500.013.2292281
GTCCTAG1501.8189894E-1212.7204111
ATTTAGA6050.012.1422111
TTTAGAA5750.012.0628832
TAGAAAT6000.012.0353434
TGTCCAC7500.011.90075210
AAATGTC6300.011.6057697
ATAGGAC2000.011.4019043
TGTCGAG1001.927483E-611.40064118
ATGTCCA6500.011.3947569
GAAATGT6350.011.3690416
AATGTCC6450.011.3358698
CAGGACT2600.011.32881454
TCCAACG951.3588797E-511.0006218
GTCCTAC19100.010.8389891
GGCGAGG8950.010.82742619
Produced by FastQC (version 0.11.5)