FastQCFastQC Report
Thu 26 May 2016
SRR1511957_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511957_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences836290
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT28270.33804063183823796No Hit
GTCCTAAAGTGTGTATTTCTCATTT22950.2744263353621352No Hit
GTCCTACAGTGGACATTTCTAAATT22650.2708390630044602No Hit
CTGTAGGACGTGGAATATGGCAAGA21500.25708785230003944No Hit
CTTTAGGACGTGAAATATGGCGAGG19120.22862882492915138No Hit
GGTATCAACGCAGAGTACTTTTTTT18350.2194214925444523No Hit
TATCAACGCAGAGTACTTTTTTTTT17990.2151167657152423No Hit
GTCCTACAGTGTGCATTTCTCATTT12690.1517416207296512No Hit
CTGAAGGACCTGGAATATGGCGAGA10420.12459792655657727No Hit
CTGTAGGACCTGGAATATGGCGAGA8740.10450920135359743No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACT500.001500065913.2982024
TAGGACC3550.013.1109024
AATCCCG604.093183E-412.66495319
GGTATCA5700.012.3479071
TAAGGGT701.0911159E-412.2126334
TTTAGTA907.3565207E-611.6241352
GGCGAGG6150.011.42934819
GTGGTAT2450.011.2581851
TTAGGGT600.00587772911.0818344
TAAGTCA951.3600418E-510.9985124
CAGTAGG1303.1997843E-810.9744921
TGGTATC2300.010.7511772
GGACCTG7100.010.7027776
AGGACCT7500.010.5119115
GTATCAA12150.010.4897471
AATGTCC3450.010.4623528
TGTAGGA11900.010.3098722
TGGCGAG13150.010.25716718
GTAGGAC12650.010.1369683
TGGGTGC856.594362E-410.05746310
Produced by FastQC (version 0.11.5)