FastQCFastQC Report
Thu 26 May 2016
SRR1511957_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511957_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences836290
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT34890.41719977519759893No Hit
GTCCTACAGTGGACATTTCTAAATT25270.30216790826148826No Hit
GTCCTAAAGTGTGTATTTCTCATTT24090.28805797032130004No Hit
CTGTAGGACGTGGAATATGGCAAGA23040.2755025170694376No Hit
GGTATCAACGCAGAGTACTTTTTTT22350.26725179064678517No Hit
TATCAACGCAGAGTACTTTTTTTTT21580.2580444582620861No Hit
CTTTAGGACGTGAAATATGGCGAGG21210.2536201556876203No Hit
GTCCTACAGTGTGCATTTCTCATTT13050.15604634755886115No Hit
ACGCAGAGTACTTTTTTTTTTTTTT10710.12806562316899642No Hit
GTACTTTTTTTTTTTTTTTTTTTTT10370.1240000478302981No Hit
CTGAAGGACCTGGAATATGGCGAGA10340.12364132059453059No Hit
CTGTAGGACCTGGAATATGGCGAGA9710.11610804864341318No Hit
TTTCTAAATTTTCCACCTTTTTCAG9280.11096629159741238No Hit
ATTTAGAAATGTCCACTGTAGGACG8570.1024764136842483No Hit
GTCCTTCAGTGTGCATTTCTCATTT8560.10235683793899245No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1400.017.727431
GGTATCA6100.013.7706141
GCCCTAA500.001445480713.3637541
TAGGACC4100.012.75056654
TGGTATC1800.012.6732912
GCCTTAC550.002958492112.1488681
CTAGAAC802.8473667E-511.8812113
AGGACCT7200.011.3531575
GTATAGG855.0629737E-511.2300461
GGCGAGG5000.011.01982619
GGACCTG6750.010.9782626
ATTATAC1053.4377463E-610.8628233
CCATACT700.001483665510.8628224
TAAGGGC700.001483665510.8628224
GTCCTAT1252.091474E-710.6910051
ACCTTTT3400.010.61113215
GTATCAA13950.010.6061541
TATACTG1451.4264515E-810.4882415
AAGGACC3450.010.469244
CTTACAC1002.3809524E-510.4554653
Produced by FastQC (version 0.11.5)