FastQCFastQC Report
Thu 26 May 2016
SRR1511956_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511956_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1411540
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT37690.2670133329554954No Hit
CTGTAGGACGTGGAATATGGCAAGA35150.24901880215934372No Hit
GTCCTAAAGTGTGTATTTCTCATTT33000.233787211131105No Hit
CTTTAGGACGTGAAATATGGCGAGG30950.21926406619720307No Hit
GTATCAACGCAGAGTACTTTTTTTT26610.18851750570299106No Hit
GGTATCAACGCAGAGTACTTTTTTT16080.11391813196933846No Hit
CTGAAGGACCTGGAATATGGCGAGA15640.11080096915425705No Hit
GTCCTACAGTGTGCATTTCTCATTT15630.11073012454482338No Hit
TATCAACGCAGAGTACTTTTTTTTT15380.10895900930898167No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG508.635593E-515.2159171
CCAACGA1101.8735591E-1013.81648119
GGTATCA7850.013.4471881
TCCAACG1450.013.10183618
GGCGAGG9200.012.802772519
TAGGACC5550.012.6651084
CGTTAGG550.00304424812.103571
ATCCCGT1103.8187864E-812.0894210
GTGCGCC1052.7233364E-711.76045711
AGGACGT18900.011.25787455
GGACGTG18900.011.1573576
GACGTGA10350.010.9213627
TTAGGAC11800.010.7868083
GTAGGAC18050.010.6828963
TCCCGTT1252.2425229E-710.6386911
GACGTGG9350.010.5655457
AAGGCGT909.524611E-510.5542566
TAGGACG20350.010.5023934
TGTAGGA17850.010.4955722
CAGTGTA4100.010.4255479
Produced by FastQC (version 0.11.5)