FastQCFastQC Report
Thu 26 May 2016
SRR1511956_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511956_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1411540
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT40560.2873457358629582No Hit
CTGTAGGACGTGGAATATGGCAAGA35670.25270272184989445No Hit
GTCCTAAAGTGTGTATTTCTCATTT34890.2471768423140683No Hit
CTTTAGGACGTGAAATATGGCGAGG32000.22670275018773822No Hit
GTATCAACGCAGAGTACTTTTTTTT30360.21508423424061665No Hit
TATCAACGCAGAGTACTTTTTTTTT18600.13177097354662284No Hit
GGTATCAACGCAGAGTACTTTTTTT17870.12659931705796507No Hit
GTCCTACAGTGTGCATTTCTCATTT17120.12128597135043993No Hit
CTGAAGGACCTGGAATATGGCGAGA16820.1191606330674299No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGCG250.0060238219.004365
GGTATCA8550.014.1756531
TCCAACG1700.013.40961418
TCCCGTT1153.8016879E-1013.21152611
TCAATAC1301.9645086E-1012.4259283
AGGACGA550.003062935812.0936845
TAGGACC5550.011.9847324
ATCCCGT1201.0073563E-811.8697310
CCAACGA1406.82121E-1011.53386419
CGTTTCC1251.8426363E-811.39494114
GGCGAGG8050.011.20938219
TATGTCG855.3459356E-511.17151216
GGACGAG600.005886879711.0803786
CCACCTT4750.010.99511913
TACACCG700.001488402610.8596345
GTCGAGG700.001493251410.85540219
TGGCGAA700.001493251410.85540218
CCGGTTT1408.401912E-910.85232512
GTCCTAA9800.010.7120371
GTCCTAC17800.010.6157491
Produced by FastQC (version 0.11.5)