FastQCFastQC Report
Thu 26 May 2016
SRR1511955_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511955_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1408562
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT30820.2188047100518117No Hit
CTGTAGGACGTGGAATATGGCAAGA30350.2154679737207166No Hit
GTATCAACGCAGAGTACTTTTTTTT27990.1987132976752177No Hit
GTCCTAAAGTGTGTATTTCTCATTT27930.1982873313350779No Hit
CTTTAGGACGTGAAATATGGCGAGG24360.17294233409675966No Hit
GGTATCAACGCAGAGTACTTTTTTT16930.1201935023094475No Hit
TATCAACGCAGAGTACTTTTTTTTT16720.1187026201189582No Hit
GTCCTACAGTGTGCATTTCTCATTT14940.10606561869481072No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5250.013.5696044
CGTGCGC655.458668E-513.15207610
GCGTGCG655.458668E-513.1520769
GGCGTGC802.0020434E-613.0607438
CGGTTTC957.397648E-812.99825113
CGGTAGG604.0522945E-412.6811691
CCGGTTT905.4125667E-712.66496312
GGTATCA6750.012.117561
GTGGTAT2650.011.8437331
GGCGAGG6450.011.63409319
TGTAGGA17600.011.4562842
AGGACCT9000.011.2929255
AGGCGTG1201.2769851E-711.0818427
AACACCG600.00588108211.0818425
GTAGGAC17800.011.0462673
CTGTAGG17900.010.99861051
GTCCTAA8500.010.9654821
GCCGGTT1053.4704462E-610.85568211
CCAACGA700.001492926410.85568219
TCCAACG1502.1354936E-910.76521818
Produced by FastQC (version 0.11.5)