FastQCFastQC Report
Thu 26 May 2016
SRR1511955_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511955_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1408562
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT35940.2551538377437415No Hit
GTCCTACAGTGGACATTTCTAAATT34770.24684749411101534No Hit
CTGTAGGACGTGGAATATGGCAAGA32870.23335856000658828No Hit
GTCCTAAAGTGTGTATTTCTCATTT28910.2052447815573613No Hit
CTTTAGGACGTGAAATATGGCGAGG26250.18636027381116343No Hit
GGTATCAACGCAGAGTACTTTTTTT21540.15292191611018896No Hit
TATCAACGCAGAGTACTTTTTTTTT20840.14795230880855795No Hit
GTCCTACAGTGTGCATTTCTCATTT15670.11124820916651167No Hit
CTGAAGGACCTGGAATATGGCGAGA14630.10386479260408843No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7800.012.6028051
TAGGACC4450.012.1721794
TCCAACG1701.0913936E-1111.1750518
GTCCTAA8350.010.9725941
CTTAGAC1605.4023985E-1010.6906983
GCCGGTT1701.4006218E-1010.61139911
TCAAGAC1355.6581484E-810.5587143
TTAGACT1303.8230428E-710.23382954
GTCCTAC15600.010.2168371
GTAGGAC16850.010.2078013
CACCTTT4750.010.19544614
AGGACGT16600.010.1325485
GGACGTG15850.010.127696
TGTAGGA17500.010.1001642
AGGACCT10450.010.0939285
GACGTGG8900.010.0288717
GTCCTAG2105.456968E-129.9983421
CCGGTTT1901.0004442E-109.99411512
CGAAATC1252.583065E-69.87558713
CCTACAG17150.09.86300853
Produced by FastQC (version 0.11.5)