FastQCFastQC Report
Thu 26 May 2016
SRR1511954_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511954_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1365772
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT37720.2761807973805291No Hit
CTGTAGGACGTGGAATATGGCAAGA37270.2728859575390329No Hit
GTCCTAAAGTGTGTATTTCTCATTT32910.24096262040809155No Hit
CTTTAGGACGTGAAATATGGCGAGG27270.19966729439467204No Hit
GTATCAACGCAGAGTACTTTTTTTT25450.18634149770239836No Hit
GTCCTACAGTGTGCATTTCTCATTT16120.11802848498870969No Hit
GGTATCAACGCAGAGTACTTTTTTT15280.11187811728458337No Hit
CTGAAGGACCTGGAATATGGCGAGA14990.10975477605339691No Hit
TATCAACGCAGAGTACTTTTTTTTT14860.10880293343252022No Hit
CTGTAGGACCTGGAATATGGCGAGA13960.10221325374952775No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA1100.015.54359819
TCCAACG1750.015.19818418
AAGCCGT400.005282853314.2482996
GTCGAGG903.643072E-813.72058419
AAGTCGT957.395647E-812.9984477
GGTATCA6850.012.494441
TGTCGAG1155.329639E-912.38982418
GCGTGCG550.003070864112.0894659
ATGTCGA1052.7229544E-711.760517
CGTAGGA657.95423E-411.7041942
TAGGACC5600.011.70395854
ACTGTTC4200.011.5343368
TCAATAC855.328012E-511.1751363
GGCTTAC1451.195076E-911.1492541
GGTATAC951.3445197E-511.0111831
TACCTAT700.001492688710.8558466
TATGTCG1053.4695677E-610.85584616
GGCGAGG6400.010.83464319
CCCTTAG803.7348055E-410.6983651
CAAGTCG1252.2421773E-710.6387296
Produced by FastQC (version 0.11.5)