FastQCFastQC Report
Thu 26 May 2016
SRR1511954_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511954_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1365772
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT41480.30371101472280876No Hit
CTGTAGGACGTGGAATATGGCAAGA37850.2771326400014058No Hit
GTCCTAAAGTGTGTATTTCTCATTT34690.25399554244778777No Hit
GTATCAACGCAGAGTACTTTTTTTT30480.22317048526401184No Hit
CTTTAGGACGTGAAATATGGCGAGG30260.22155967467483592No Hit
GTCCTACAGTGTGCATTTCTCATTT18300.13399015355418034No Hit
GGTATCAACGCAGAGTACTTTTTTT18010.13186681232299388No Hit
TATCAACGCAGAGTACTTTTTTTTT17880.1309149697021172No Hit
CTGAAGGACCTGGAATATGGCGAGA16570.12132332483020593No Hit
CTGTAGGACCTGGAATATGGCGAGA14220.10411693899128112No Hit
GTCCTTCAGTGTGCATTTCTCATTT13950.10214003508638339No Hit
ATTTAGAAATGTCCACTGTAGGACG13700.10030956850777435No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGAGA350.002178078816.2766768
GGCGTGC755.8835212E-815.1915658
GTGCGCC755.8835212E-815.19156511
TAAGACG400.005263493414.2561854
GCGTGCG759.730938E-713.9256019
TACACCG500.001494489613.3057735
GGTATCA6000.013.040581
AGGCGTG957.437848E-812.9927857
TAGGACC5500.012.7873654
GGCGAGG7950.012.66566619
CGGTTTC1052.0070729E-812.66010113
CGTGCGC751.48673225E-512.65963710
GCGCCAC853.9652423E-612.287744513
GTCCTAA8750.012.2136151
AAGGCGT1103.8180588E-812.089516
TGCGCCA1103.8415237E-812.084212
GAGTGCG658.059771E-411.6858197
CTAGGAC2050.011.5903933
AATACCC1001.9187792E-611.4049475
TTAGGAC10650.011.3335543
Produced by FastQC (version 0.11.5)