FastQCFastQC Report
Thu 26 May 2016
SRR1511953_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511953_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1475184
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT33370.2262090695126845No Hit
CTGTAGGACGTGGAATATGGCAAGA28220.19129817026215035No Hit
GTCCTAAAGTGTGTATTTCTCATTT26980.18289243918046832No Hit
CTTTAGGACGTGAAATATGGCGAGG24180.16391175609279926No Hit
GTATCAACGCAGAGTACTTTTTTTT22350.15150652393192984No Hit
GTCCTACAGTGTGCATTTCTCATTT18430.12493356760919316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA1200.015.03962819
CGTAGGA400.00523869714.2664432
TCCAACG1750.014.11237118
GGTATCA6800.013.5671081
TAGGACC6500.013.2981974
GGAAACG604.0964904E-412.66494917
AGGACGA1001.4447687E-712.3483265
CGGTAGG701.079146E-412.228381
GTGCGCC802.8701086E-511.87338911
TGTACTG2300.011.5636495
AATCCCG2550.011.54745319
CCGGTTT1657.2759576E-1211.51359112
GGCGAGG6300.011.00644419
TGTAGGA17300.010.9953322
GGCGTGC1053.47085E-610.8556728
AGGACGG700.001493011810.8556715
ACTGTTC6100.010.7444458
GTAGGAC18550.010.7020523
GACGTGG8800.010.6860517
GAAACGG803.7751562E-410.6860518
Produced by FastQC (version 0.11.5)