FastQCFastQC Report
Thu 26 May 2016
SRR1511953_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511953_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1475184
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT34930.23678402151867156No Hit
CTGTAGGACGTGGAATATGGCAAGA31560.21393941365958416No Hit
GTCCTAAAGTGTGTATTTCTCATTT28540.1934673911864554No Hit
CTTTAGGACGTGAAATATGGCGAGG27030.183231379949891No Hit
GTATCAACGCAGAGTACTTTTTTTT24580.16662328224818057No Hit
GTCCTACAGTGTGCATTTCTCATTT19580.13272920530591437No Hit
TATCAACGCAGAGTACTTTTTTTTT15710.10649518975260036No Hit
GGTATCAACGCAGAGTACTTTTTTT15260.1034447228277964No Hit
CTGTAGGACCTGGAATATGGCGAGA15120.10249568867341295No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGAT400.005298217314.2421711
ACCGTGC551.9686374E-413.8110588
GGTATCA6050.012.9298761
TAGGACC4800.012.8697294
GACCGTG751.486321E-512.6601367
ATAGGAC1850.012.3292543
CCAACGA1603.6379788E-1211.87451319
GGCGAGG7000.011.67094919
TAGGACA5800.011.6339634
CCGTGCT855.3502292E-511.1707099
GTCGAGG600.00587721211.08287919
TCCAACG2400.011.08287918
CGTGCAC600.00589950211.07724310
ATTATAC1900.011.0043993
GGACGTG16500.010.8806336
TGTAGGA16750.010.7804292
TGGCGAG15350.010.76828818
GTAGGAC16400.010.66283
TTAGGAC10800.010.6477753
CTGTAGG16500.010.638241
Produced by FastQC (version 0.11.5)