FastQCFastQC Report
Thu 26 May 2016
SRR1511952_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511952_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1189996
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT46780.3931105650775296No Hit
CTGTAGGACGTGGAATATGGCAAGA44910.37739622654193794No Hit
GTCCTAAAGTGTGTATTTCTCATTT40640.341513752987405No Hit
CTTTAGGACGTGAAATATGGCGAGG37460.31479097408730783No Hit
GTATCAACGCAGAGTACTTTTTTTT22590.18983257086578442No Hit
GTCCTACAGTGTGCATTTCTCATTT20680.17378209674654368No Hit
CTGAAGGACCTGGAATATGGCGAGA19700.16554677494714268No Hit
ATTTAGAAATGTCCACTGTAGGACG17090.14361392811404408No Hit
GTCCTTCAGTGTGCATTTCTCATTT15160.12739538620297883No Hit
TTTCTAAATTTTCCACCTTTTTCAG15160.12739538620297883No Hit
GAATATGGCAAGAAAACTGAAAATC13980.11747938648533274No Hit
CTGTAGGACCTGGAATATGGCGAGA13610.11437013233657929No Hit
GGAATATGGCGAGAAAACTGAAAAT13290.11168104766738711No Hit
GGTATCAACGCAGAGTACTTTTTTT12440.10453816651484543No Hit
TATCAACGCAGAGTACTTTTTTTTT12310.1034457258679861No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG851.0040822E-915.662371
CTCGAAC953.0559022E-1014.99827518
TCGAACT902.2901077E-914.77607919
GGTATCA5300.013.4565521
TAGGACC6450.013.401564
CCACCTT5500.013.298470513
ACCTTTT6000.012.82352515
CACCTTT5900.012.55787814
GGCCTAT550.003045327312.102741
TTCCACC5900.011.91388311
TCCACCT6300.011.91132912
ACTGTTC5150.011.8044678
GGCCTAC752.0542878E-411.4111561
TTAGGAC13750.011.3986883
TAGAAAT6150.011.2751264
GGCGAGG10400.011.23428419
GGCCTCG1104.971225E-711.22598215
CCAACGT855.326435E-511.17518519
CTAGGGC600.00587931911.0820593
TTTCCAC4850.010.96781110
Produced by FastQC (version 0.11.5)