FastQCFastQC Report
Thu 26 May 2016
SRR1511952_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511952_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1189996
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT47080.39563158195489734No Hit
CTGTAGGACGTGGAATATGGCAAGA45080.3788248027724463No Hit
GTCCTAAAGTGTGTATTTCTCATTT42000.3529423628314717No Hit
CTTTAGGACGTGAAATATGGCGAGG39240.3297490075596893No Hit
GTATCAACGCAGAGTACTTTTTTTT27990.23521087465840218No Hit
GTCCTACAGTGTGCATTTCTCATTT21750.18277372360915498No Hit
CTGAAGGACCTGGAATATGGCGAGA20640.17344596116289468No Hit
ATTTAGAAATGTCCACTGTAGGACG17160.14420216538542988No Hit
TATCAACGCAGAGTACTTTTTTTTT16340.13731138592062495No Hit
TTTCTAAATTTTCCACCTTTTTCAG16220.13630297916967787No Hit
GTCCTTCAGTGTGCATTTCTCATTT15680.13176514879041612No Hit
GGTATCAACGCAGAGTACTTTTTTT15580.13092480983129356No Hit
GAATATGGCAAGAAAACTGAAAATC15040.12638697945203178No Hit
CTGTAGGACCTGGAATATGGCGAGA13920.1169751831098592No Hit
GGAATATGGCGAGAAAACTGAAAAT13040.10958020026958075No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6200.012.8765534
GGTATCA6700.012.3943561
GGCGAGG10100.012.22788419
GTATTAA2000.011.9313481
TCCAACG1800.011.61126518
TAAGGGT1104.936064E-711.2321444
CCACCTT5600.011.19045913
TAGGAAC600.00585534311.0881434
CGGTTTC1201.283297E-711.07742513
GAACAGT3950.011.0629736
GGACCTG12550.011.0514766
TAGGACA5400.010.9121414
AGGACCT12850.010.872425
AGGACGG700.001485569210.8618555
AGGACGA700.001485569210.8618555
CCAACGA1158.80651E-710.73927319
TTAAGCA1355.6434146E-810.5601364
TGGCGAG22900.010.45428818
CTGTAGG21850.010.4406121
TGTAGGA22600.010.4293022
Produced by FastQC (version 0.11.5)