FastQCFastQC Report
Thu 26 May 2016
SRR1511951_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511951_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1216891
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT30530.250885247733774No Hit
CTGTAGGACGTGGAATATGGCAAGA29700.24406458754317353No Hit
GTCCTAAAGTGTGTATTTCTCATTT26530.21801459621280786No Hit
GTATCAACGCAGAGTACTTTTTTTT23040.18933495276076492No Hit
CTTTAGGACGTGAAATATGGCGAGG22130.18185687953974514No Hit
GTCCTACAGTGTGCATTTCTCATTT14330.11775910907386117No Hit
GGTATCAACGCAGAGTACTTTTTTT14250.11710169604344187No Hit
TATCAACGCAGAGTACTTTTTTTTT14220.11685516615703462No Hit
CTGAAGGACCTGGAATATGGCGAGA13470.11069191899685346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGC707.168961E-613.5879161
GTATTAG801.9713225E-613.078371
GGTATCA5700.013.0157931
GATATAC1950.012.6820551
TCCAACG802.8693501E-511.87330518
GATTTAA855.2550196E-511.1900491
GCCGGTT600.0058806611.08175211
AGACGGT600.0058806611.0817526
GGACCGT600.0058806611.0817526
TGTAGGG1553.0559022E-1011.0456622
TAGGACC5100.010.9886274
TTATACT1303.268906E-810.9599734
AAATGTC4000.010.9234417
GGCGAGG6150.010.81146419
GTCCTAC13700.010.7612331
GTCCTAG2300.010.7521771
GACCTAC909.393899E-510.5683791
CCTACAG15500.010.4178683
GTCCTAA6850.010.2752421
GGACCTG9400.010.2059916
Produced by FastQC (version 0.11.5)