FastQCFastQC Report
Thu 26 May 2016
SRR1511951_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511951_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1216891
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT35110.28852214372528023No Hit
CTGTAGGACGTGGAATATGGCAAGA33130.272251171222402No Hit
GTCCTAAAGTGTGTATTTCTCATTT28110.2309985035635895No Hit
GTATCAACGCAGAGTACTTTTTTTT27450.22557484606263012No Hit
CTTTAGGACGTGAAATATGGCGAGG24280.19952485473226445No Hit
GGTATCAACGCAGAGTACTTTTTTT16780.13789238313045293No Hit
GTCCTACAGTGTGCATTTCTCATTT16190.13304396203111044No Hit
TATCAACGCAGAGTACTTTTTTTTT15470.12712724475733653No Hit
CTGAAGGACCTGGAATATGGCGAGA13690.1124998048305066No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGCA350.002179388216.2750079
GTCGAGG704.4295302E-714.92979919
CCGCAAG400.005299865714.24121615
AAGTCGT1051.382432E-913.5625067
TGTCGAG1001.0057192E-813.30054718
ATGTCGA1001.0149961E-813.29180117
TATGTCG1001.0149961E-813.29180116
TCCAACG801.9974595E-613.06303718
CAAGTCG957.381459E-813.06
GGTATCA5150.012.6058861
TGTAGGA15450.011.3195022
AGGACGT16350.011.2777365
ACTGTTC2950.011.2637778
GCCGGTT855.356183E-511.16912311
TACACCG600.00585287311.0888045
CTGTCCG600.005903242611.0760469
GGACGTG15950.011.0188096
CTGTAGG15100.011.0013011
GTAGGAC15300.010.9956223
GCCCTAG1053.2642292E-610.9109781
Produced by FastQC (version 0.11.5)