FastQCFastQC Report
Thu 26 May 2016
SRR1511950_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511950_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1589330
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT19140.12042810492471671No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTGT250.00603640918.9976718
GTACCGA550.003071314712.0894286
GGTATCA6000.011.2537431
AGGACGT8100.011.023345
GTCCTAC8200.011.01792051
TAGGACG8150.010.8391624
GGACGTG7750.010.7857756
GTCCTAA4650.010.6350681
CCTAGAC1451.4455509E-810.4814743
GTATCAA12050.010.3388951
GTATTAC750.002624147610.1442191
TCCTACA9100.010.1366142
GACGTGG3750.010.13209157
GTGTAGA2400.09.9064641
ACGTGGA4050.09.8506448
TTATACT2800.09.838084
CTAGACT1451.6191916E-79.8263824
GACTAGA1553.994137E-89.8169861
GGCGAGG4300.09.71973919
GCCTTAG1304.1794465E-69.5102051
Produced by FastQC (version 0.11.5)