FastQCFastQC Report
Thu 26 May 2016
SRR1511950_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511950_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1589330
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT23740.1493711186474804No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAATC456.784817E-414.77134115
GCGTGCG400.00529776614.242459
CCGTATG655.479819E-513.1468779
GGCGAGG3850.012.33655819
ACCGTAT701.0963414E-412.2078128
GGTATCA5700.011.8902841
CGTGCGC658.061476E-411.68574410
GTATCAA13250.011.6709941
ACGTGAA5150.011.6152018
TACACCG1157.050767E-811.5678245
CGTGAAA5350.011.1809889
GGACGTG8250.011.1683466
GTGTCCG600.005898983711.077469
GACGTGA5100.010.9843737
TTAGGAC5900.010.951623
CTAGGCT1752.0008883E-1110.859594
AGGACGT8900.010.7833295
GTACTAA1355.2985342E-810.6063561
TGGCGAG7150.010.49556418
TGCTCGG1002.4133737E-510.44413410
Produced by FastQC (version 0.11.5)