FastQCFastQC Report
Thu 26 May 2016
SRR1511949_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511949_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1599930
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT22660.14163119636484098No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGCC1001.0091753E-813.2981411
TAGGACC3650.012.7512444
TGAACCG701.09268265E-412.2121965
ACCGTCC550.003071729612.0892188
CAGAACG951.0419753E-611.9979484
GGCCTAC1156.952723E-811.5795231
CGTGCGC752.0765826E-411.39840610
GCACCGT855.3304415E-511.1749086
TCGTGCA600.00588190311.0817849
CCATCGC600.00588190311.08178419
TAGGACA4250.010.7275764
GGACGTG8600.010.7136176
GGTATCA5600.010.7007211
GTAGGAC10050.010.6797653
AGGACGT8950.010.6127095
GGCGAGG3800.010.49853219
GTATCAA12200.010.4473331
GACGTGA4650.010.2136257
TAGGACG9700.010.18382554
GTCCTAA5050.010.1709811
Produced by FastQC (version 0.11.5)