FastQCFastQC Report
Thu 26 May 2016
SRR1511949_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511949_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1599930
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT26700.16688230110067315No Hit
GTCCTACAGTGGACATTTCTAAATT16900.1056296212959317No Hit
TATCAACGCAGAGTACTTTTTTTTT16240.10150444081928585No Hit
GGTATCAACGCAGAGTACTTTTTTT16070.10044189433287705No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTAAA657.7138044E-411.74731351
GGTATCA7350.011.6873781
CGTGCGC1157.1322575E-811.55825410
TAGGACC3650.011.4569764
GCACCGT752.0752502E-411.3991936
GCGTGCG1001.9435338E-611.3931379
GTCCTAT1053.2762528E-610.9082191
CTTATAC1451.43318175E-810.4872653
TGTAGGA9000.010.3488892
CTATAAG1201.4342841E-610.3400831
GGACGTG7850.010.2858956
AGGACGT7850.010.1699735
GTAGGAC8800.010.1520893
TAGCACC750.002638222410.1376884
CCATCGC750.002648510710.13293319
CTAGACA1701.546141E-910.0631474
CCCTTAC951.5678308E-410.0470451
TGGTATC2850.010.0042972
TTAGACT1054.0850777E-59.9566574
GGCGTGC1252.5890258E-69.8740528
Produced by FastQC (version 0.11.5)