FastQCFastQC Report
Thu 26 May 2016
SRR1511948_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511948_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1045611
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT24250.2319218141354672No Hit
GGTATCAACGCAGAGTACTTTTTTT14890.14240477577225183No Hit
TATCAACGCAGAGTACTTTTTTTTT14370.1374316069742954No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5100.014.35788251
CGTGCGC500.001500340613.29839210
GCGTGCG500.001500340613.2983929
GTATTAG655.3968717E-513.1673691
TAGACAG1001.4429315E-712.3485085
GTATAGG550.003043774312.1033391
CTAAGAC855.325317E-511.175123
GCCTAAC600.005828722411.09472751
TTAGGGT700.00149219910.8558314
GTATCAA11700.010.7289671
CTCATAC1201.5237893E-610.2904243
GGTTTAA750.00262386710.1437511
CTAGACA750.002648985510.132114
TTAACCT750.002648985510.132114
TAGAGCT1151.0265827E-59.9118454
TAGAACC1252.5699646E-69.8788064
TGAGGTG1651.02136255E-89.7866965
GTACAAA1951.6007107E-109.7536071
GTAGGGG800.00451786359.4988533
GACTACT800.00451786359.4988537
Produced by FastQC (version 0.11.5)