FastQCFastQC Report
Thu 26 May 2016
SRR1511947_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511947_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1589374
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT21630.13609131645540948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCCC802.002309E-613.0608564
GGTATCA7450.012.7653341
CGTGCGC751.4805471E-512.66507310
GCGTGCG604.0964643E-412.6650729
GCGTGCA658.0320734E-411.6908369
TCGAACT1104.9759365E-711.22586119
CGTTTAA600.005829130811.09520151
CTCGAAC1303.2712705E-810.96015818
CCATTCG700.001493001110.8557779
TAGGACC2750.010.7077434
ATGCCGT803.7751626E-410.6861556
GCCTCGA1252.2433778E-710.63866116
CTAGACC909.526611E-510.5542273
GTCCTAA4000.010.4611911
GGCGTGC1002.4017989E-510.4486858
GGACGTG7100.010.301526
AGGACGT7350.010.20959955
CGAGCAA750.002650393210.13205810
TAGACAA1606.180926E-910.092485
GGGTATA856.528553E-410.0695951
Produced by FastQC (version 0.11.5)