FastQCFastQC Report
Thu 26 May 2016
SRR1511947_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511947_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1589374
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT25600.16106970417283786No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC500.001506909513.29138910
GGTATCA8150.012.2982741
TTGCACG550.003058079212.09644
CTAGACT1552.5465852E-1111.650454
GGCGAGG3750.011.14541119
ACGACAG600.00585592811.0883675
GTATAAG951.2895098E-511.0530341
GTTCTAA1158.285497E-710.7909071
TGGCGAG7650.010.678534518
GTGGTAT3150.010.6064471
TAATACC1355.6475983E-810.5603494
TATGGCG8050.010.38000216
GTATCAA17350.010.3435781
ATTTAGA2400.010.3412871
ATGGCGA8050.010.26204817
GTGCTAA1409.026553E-810.2276451
AAGGCGT750.002648718410.132836
CCTTATA1701.546141E-910.0633932
GTTATAT1053.893031E-510.0003641
GTCTAAG1356.0300226E-79.8993511
Produced by FastQC (version 0.11.5)