FastQCFastQC Report
Thu 26 May 2016
SRR1511946_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511946_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences734182
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT21170.2883481207656957No Hit
CTGTAGGACGTGGAATATGGCAAGA17510.23849672152136664No Hit
GTCCTACAGTGGACATTTCTAAATT16780.22855368287427366No Hit
GTCCTAAAGTGTGTATTTCTCATTT15770.21479687597898067No Hit
CTTTAGGACGTGAAATATGGCGAGG13270.18074537376290892No Hit
GGTATCAACGCAGAGTACTTTTTTT13030.17747642955016604No Hit
TATCAACGCAGAGTACTTTTTTTTT12640.17216439520445884No Hit
GTCCTACAGTGTGCATTTCTCATTT8530.11618372556123685No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGAC508.711954E-515.1983643
GCTAGAC707.2581242E-613.569973
GTATAAG1001.4213401E-712.36129951
ATAAGAC853.9400475E-612.2927963
GGTATCA3100.012.2692811
CTAGTAC550.003068264812.089613
ATAATAC951.0386284E-611.998713
CCTCTAA657.9533085E-411.7030061
GTATCAA7600.011.5105231
TAGGACA3100.011.337494
CTAGGAC1354.7148205E-911.2580483
TAGGACC2800.011.1952254
CTTATCC600.00587535711.0821423
TTACACC600.00587535711.0821424
GTAGTAC600.00587535711.0821423
AGGACCT5850.011.0415485
GTCTAGG1053.4197947E-610.8670771
AGACAGT1408.3273335E-910.8559766
GGCGAGG3800.010.74884419
CTTACAC803.7693142E-410.686353
Produced by FastQC (version 0.11.5)