FastQCFastQC Report
Thu 26 May 2016
SRR1511946_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511946_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences734182
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT27350.3725234342438251No Hit
TATCAACGCAGAGTACTTTTTTTTT18810.25620350267372394No Hit
GGTATCAACGCAGAGTACTTTTTTT17760.2419018717429738No Hit
CTGTAGGACGTGGAATATGGCAAGA16210.2207899403690093No Hit
GTCCTACAGTGGACATTTCTAAATT15780.214933081987845No Hit
GTCCTAAAGTGTGTATTTCTCATTT15450.21043828369532352No Hit
CTTTAGGACGTGAAATATGGCGAGG13550.18455914201110896No Hit
ACGCAGAGTACTTTTTTTTTTTTTT9400.12803364833242983No Hit
GTCCTACAGTGTGCATTTCTCATTT8980.12231299596012979No Hit
CTGAAGGACCTGGAATATGGCGAGA8570.11672854959669401No Hit
GTACTTTTTTTTTTTTTTTTTTTTT7480.10188209463048672No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTTC350.002175616.2780113
TTTGCAC551.9510924E-413.8238833
GGTATCA5750.013.2689781
TAATACC604.073252E-412.6718934
TGAGCCG604.073252E-412.6718935
ACTGTTC1352.7284841E-1112.6598118
TAGGACC2550.012.299194
GGCGAGG4450.011.95591419
ATAATAC802.8494744E-511.87993
GTGGTAT1950.011.7379421
TGTAGGA9650.011.6213732
TGGTATC2000.011.4047032
TGTTCTA1501.7644197E-1011.3938310
CACCTTT2600.011.32156414
TAATAGC600.005852650411.0879074
TAATACA600.005852650411.0879074
TGAACCA600.005852650411.0879075
GGACGTG10550.011.0751146
CTAGGAC951.3511808E-511.0045393
CTGTTCT2250.010.9718379
Produced by FastQC (version 0.11.5)