FastQCFastQC Report
Thu 26 May 2016
SRR1511945_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511945_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1584856
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT40360.25466036031033734No Hit
CTGTAGGACGTGGAATATGGCAAGA37800.23850747323416133No Hit
GTCCTAAAGTGTGTATTTCTCATTT36200.22841191881155135No Hit
CTTTAGGACGTGAAATATGGCGAGG32810.20702196287864638No Hit
GTATCAACGCAGAGTACTTTTTTTT29610.1868308540334264No Hit
CTGAAGGACCTGGAATATGGCGAGA18400.11609887586001505No Hit
GTCCTACAGTGTGCATTTCTCATTT17630.11124039029413399No Hit
GGTATCAACGCAGAGTACTTTTTTT17110.10795933510678574No Hit
TATCAACGCAGAGTACTTTTTTTTT16880.10650809915853554No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGCA350.002172248216.2834427
CGTTAGG456.700266E-414.7957891
TAGGACC5850.014.2886054
CGGTAGG759.54109E-713.95031451
TCCAACG1351.8189894E-1213.36850518
GGTATCA7400.012.4678791
TCGAACT951.041557E-611.99832619
AATCCCG2800.011.87334319
AGGACCT12950.011.6624655
GCGCCAC600.005881848711.08178713
GGACCTG13000.010.74080856
GTCTAAT803.7285555E-410.7005251
CCGTTTC1252.2437962E-710.63851513
AAATCCC3500.010.58423718
GTATCAA15250.010.54071
GTGTAGA2800.010.5306761
GGCGAGG8850.010.51830819
GACGTGA11200.010.516397
GGACGTG20450.010.4508656
CTAGTAC1002.4021496E-510.4485433
Produced by FastQC (version 0.11.5)