FastQCFastQC Report
Thu 26 May 2016
SRR1511945_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511945_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1584856
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT44860.283054107123928No Hit
CTGTAGGACGTGGAATATGGCAAGA42020.26513449802379524No Hit
GTCCTAAAGTGTGTATTTCTCATTT38800.24481719474829258No Hit
GTATCAACGCAGAGTACTTTTTTTT36260.22879050210239923No Hit
CTTTAGGACGTGAAATATGGCGAGG35930.2267082940027359No Hit
GGTATCAACGCAGAGTACTTTTTTT21980.13868767888060493No Hit
TATCAACGCAGAGTACTTTTTTTTT21350.13471255432670223No Hit
GTCCTACAGTGTGCATTTCTCATTT18970.11969541712306986No Hit
CTGAAGGACCTGGAATATGGCGAGA18760.11837037560510229No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCTC350.002177677816.2773598
TATTCCG400.005265096714.2557325
GGTATCA8400.013.7442791
GACTGCG655.4789372E-513.1470977
TAGGACC5900.012.5643734
CTAATAC1302.5975169E-911.697013
GTGGTAT2650.011.52179051
TACTAGA700.001486435110.8615092
TAGGACA5100.010.8082674
GGACGTG22550.010.5731916
TGTAGGA22050.010.5598012
ATGTCCA6300.010.550149
AGGACGT23150.010.50962455
GGCGAGG10650.010.43554519
AATGTCC6100.010.4290728
GTAGGAC21200.010.4004083
GTCCTAA10350.010.3250841
CTGTAGG21750.010.265321
TTAGGAC14100.010.2452543
GACGTGG10850.010.2389847
Produced by FastQC (version 0.11.5)