FastQCFastQC Report
Thu 26 May 2016
SRR1511944_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511944_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1495857
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT31410.20997996466239754No Hit
CTGTAGGACGTGGAATATGGCAAGA28820.19266547537632273No Hit
GTCCTAAAGTGTGTATTTCTCATTT27120.18130075267889914No Hit
GTATCAACGCAGAGTACTTTTTTTT24480.1636520068429001No Hit
CTTTAGGACGTGAAATATGGCGAGG24160.16151276492338507No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCT250.006036511618.9975287
CGGTAGG602.5414538E-514.2657891
CTGATCG400.005283542414.2481449
CTAATTG500.001500973213.2982693
TAGGACC4850.012.9261524
GGTATCA6450.012.238351
GTACCGT600.00588117911.081896
TACACGG700.001492966510.8557295
GGCGAGG6100.010.58878519
GCCGGTT909.5261785E-510.55418111
TTAGTAC909.5261785E-510.5541813
GTCCTAA7700.010.4986321
TCTTATA1451.4177203E-810.4943732
TTATAGT1451.445369E-810.4813954
GTAGGAC16600.010.4715293
GTATTAG1105.9642225E-610.3751181
TAGAAAT4250.010.28101354
TATACTG2800.010.1772465
CCCCTAC750.00262326110.1445611
GCCCTAA750.00262326110.1445611
Produced by FastQC (version 0.11.5)