FastQCFastQC Report
Thu 26 May 2016
SRR1511944_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511944_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1495857
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT33910.22669279215860877No Hit
CTGTAGGACGTGGAATATGGCAAGA30270.20235891532412523No Hit
GTATCAACGCAGAGTACTTTTTTTT29210.19527267646573168No Hit
GTCCTAAAGTGTGTATTTCTCATTT27830.1860471956878231No Hit
CTTTAGGACGTGAAATATGGCGAGG27260.18223667101868696No Hit
GGTATCAACGCAGAGTACTTTTTTT16550.11063891802491817No Hit
TATCAACGCAGAGTACTTTTTTTTT16500.11030466147499395No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTACG500.001494415913.3059972
GGTATCA8200.012.4561121
TTAGACT1302.5956979E-911.6975794
TAGGGCC657.992827E-411.6975794
GGCGAGG6400.011.57598619
TGTACCG1104.938247E-711.2323345
GTACCGC600.0058806411.08202656
CCGTGCC600.00589969611.077219
TAGGACC4050.011.0296624
GACGTGG7750.011.0261637
CTAATAC1303.2428943E-810.966483
GGACGTG15850.010.84730156
GTCTATA1252.0955122E-710.6913021
AGGACGT16450.010.6887085
GTGGTAT3350.010.5431291
GTCCTAC14700.010.454931
TGTAGGA15350.010.40208152
TAGACTC1106.011136E-610.3683095
GTCCTAA8000.010.2617421
CGTGGAA8450.010.2251199
Produced by FastQC (version 0.11.5)