FastQCFastQC Report
Thu 26 May 2016
SRR1511943_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511943_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1006278
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT40980.4072433263968804No Hit
GGTATCAACGCAGAGTACTTTTTTT23520.2337326265703911No Hit
TATCAACGCAGAGTACTTTTTTTTT22780.22637879393169683No Hit
ACGCAGAGTACTTTTTTTTTTTTTT10350.10285428082498077No Hit
GGTATCAACGCAGAGTACATGGGGG10110.10046925402324208No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7000.012.4990521
GTATAAT2300.012.4045421
TTCAACG550.003042720712.1038262
GTCTATA1104.8928996E-711.2392661
GGTATAA600.00582672211.0951731
GTGGTAT2250.010.9895051
ACCGTGC700.001492172610.855788
GTATCAA15650.010.5128161
GACAGTA1002.39939E-510.4486887
GTCTAGA1201.5005153E-610.3026611
GACTAAA750.002622844710.1441591
TTTAGGC856.5962505E-410.0575613
TGGTATC2650.010.0484592
CTCCTAA951.6249095E-410.0106831
CCTAAAC1151.026517E-59.9117993
TTAGGAC1356.4411506E-79.85061553
TAATGGA2800.09.8380514
GTCCTAT800.00447353269.5101491
ATGTAAC900.00110197859.5101491
GTATAAA900.00110197859.5101491
Produced by FastQC (version 0.11.5)