FastQCFastQC Report
Thu 26 May 2016
SRR1511943_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511943_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1006278
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT49480.49171302562512553No Hit
GGTATCAACGCAGAGTACTTTTTTT29510.2932589204971191No Hit
TATCAACGCAGAGTACTTTTTTTTT28920.2873957296095115No Hit
ACGCAGAGTACTTTTTTTTTTTTTT13840.1375365455669308No Hit
GGTATCAACGCAGAGTACATGGGGG10980.10911497617954481No Hit
GTACTTTTTTTTTTTTTTTTTTTTT10290.1022580241245461No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGA250.0060337318.998316
GGTATCA6950.013.7361461
TCTACGG550.003054366712.0976532
GTATTAC907.0576916E-611.6680931
TAGGACA907.4069994E-611.6175874
GTATCAA17600.011.3366141
TACGGAT600.005848886411.0895154
GTATGAT951.28697175E-511.0539841
ACCCTAT700.001430940310.9104251
TAGTACT1002.3813609E-510.4558294
CTAGAAC1201.5103669E-610.2974063
TCTAGGA1502.417437E-810.1389862
CAAACCC856.554938E-410.0644344
CCTAATA856.554938E-410.0644342
TCTATAC951.6332947E-410.0055783
TCTATGG1054.075245E-59.95793152
GTATATA2251.8189894E-129.7587691
GTGGTAT2750.09.7201981
GTATAAA1303.975447E-69.5466221
TGTATCA1002.7372898E-49.50532
Produced by FastQC (version 0.11.5)