FastQCFastQC Report
Thu 26 May 2016
SRR1511942_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511942_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1135251
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT37210.32776892510995365No Hit
GTCCTACAGTGGACATTTCTAAATT25780.22708634478190284No Hit
CTGTAGGACGTGGAATATGGCAAGA23960.21105464782678018No Hit
GGTATCAACGCAGAGTACTTTTTTT23740.20911675039264446No Hit
TATCAACGCAGAGTACTTTTTTTTT21870.1926446222024909No Hit
GTCCTAAAGTGTGTATTTCTCATTT17960.15820289962307896No Hit
CTTTAGGACGTGAAATATGGCGAGG17880.1574982096470296No Hit
GTCCTACAGTGTGCATTTCTCATTT14150.12464203951372868No Hit
CTGTAGGACCTGGAATATGGCGAGA11610.10226813277416184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5200.014.0651564
TGTCGAG551.9619647E-413.81606718
AATCCCG1001.0082658E-813.29796519
GGTATCA7400.012.8545571
CGGTAGG701.0772311E-412.2301931
GTATTAC550.00303781112.1066551
CGTAGGA550.00303781112.1066552
GTGGTAT2550.011.9370951
TCCAACG802.8692557E-511.87318318
GGTCAAT1001.8979263E-611.4148461
AAATGTC3700.011.2955687
GGACCTG9300.011.1327056
AGGACCT9750.011.1059925
GCCGGTT600.00588069811.08163711
GTTAGAC700.001492769710.8554823
TATGTCG700.001492769710.85548216
CCACCTT3150.010.85548113
ACCTTTT3350.010.7744715
TGGTATC2400.010.7014182
GTAGGAC14750.010.689893
Produced by FastQC (version 0.11.5)