FastQCFastQC Report
Thu 26 May 2016
SRR1511942_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511942_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1135251
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46490.4095129623316782No Hit
GGTATCAACGCAGAGTACTTTTTTT30000.26425874101850605No Hit
GTCCTACAGTGGACATTTCTAAATT28920.25474542634183983No Hit
CTGTAGGACGTGGAATATGGCAAGA26610.23439750328341485No Hit
TATCAACGCAGAGTACTTTTTTTTT26370.2322834333552668No Hit
GTCCTAAAGTGTGTATTTCTCATTT19210.16921368049885002No Hit
CTTTAGGACGTGAAATATGGCGAGG19110.16833281802878836No Hit
GTCCTACAGTGTGCATTTCTCATTT14670.12922252435804946No Hit
ACGCAGAGTACTTTTTTTTTTTTTT13300.11715470851820435No Hit
CTGTAGGACCTGGAATATGGCGAGA12170.10720096260650729No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACCGT655.4503147E-513.1537546
GGTATCA9150.013.1415281
TAGGACC4300.013.0416894
TCCAACG1051.9927029E-812.66601918
TCGAACT550.003067727712.09082419
GGCGAGG6550.012.03808419
TCTAAGC657.985443E-411.6984133
GACGTGA7600.011.3679087
GGACGTG13850.011.3176116
GTGGTAT3050.011.2641671
TTAGGAC8950.011.2572723
CTTACTC1104.9296796E-711.2331353
AGGACGT14000.011.1343825
GTCCTAA6800.011.0870151
TGGTATC2850.011.0057432
GATATAC2900.010.8595641
ACTGTTC3250.010.8086418
GTTCTAA1158.3060877E-710.7880251
TAGGACG14350.010.7303394
TTGGACC803.7508443E-410.693084
Produced by FastQC (version 0.11.5)