FastQCFastQC Report
Thu 26 May 2016
SRR1511941_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511941_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1842137
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT24140.1310434565941621No Hit
GTCCTACAGTGGACATTTCTAAATT23680.12854635675848214No Hit
CTGTAGGACGTGGAATATGGCAAGA19750.10721243859712931No Hit
GTCCTAAAGTGTGTATTTCTCATTT18830.10221823892576937No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAT401.2867198E-518.9977748
TACACCG853.9496026E-612.2926785
GTCCTAA6200.011.9635271
ACCGTCC802.8704748E-511.8736098
TAGGACC4750.011.7986174
GGCGAGG5700.011.665319
ACACCGT1157.0871465E-811.5638626
GTCCTAT1604.5474735E-1111.2924961
GGTATCA8800.011.022341
GGACGTG9700.010.576086
TTAGGAC7850.010.527433
GACGTGA5700.010.498777
TAGGACG10850.010.3305874
TGGCGAG12700.010.32162518
TCCAACG1201.5260921E-610.29046218
AGGACGT10800.010.2025095
GTACCGT750.00265054510.1321466
TGTAGGA12550.010.0777652
GTAGGAC12350.010.0757433
ACGTGAA6850.09.9842328
Produced by FastQC (version 0.11.5)