FastQCFastQC Report
Thu 26 May 2016
SRR1511941_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511941_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1842137
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT29820.16187721108690614No Hit
GTCCTACAGTGGACATTTCTAAATT26010.14119471027399158No Hit
CTGTAGGACGTGGAATATGGCAAGA22930.12447499833074303No Hit
GTCCTAAAGTGTGTATTTCTCATTT18850.1023268084838424No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG751.4146641E-512.7215131
GGTATCA7600.012.1775011
TAGGACC4350.012.0166314
GCGTGCG802.8832237E-511.8684679
GGCGAGG4550.011.69317619
TATACTG2500.011.4048745
GTATCAA17050.011.1919471
GCGCCAC951.3679599E-510.99454613
TCCAACG1053.463656E-610.8579518
GCTAGAC909.475184E-510.5600693
GTCCTAC12850.010.5435111
GTCCTAA5800.010.5281481
ATAGGAC2001.8189894E-1210.4544693
GGACGTG11950.010.4127376
TTACACC2100.010.4092114
CGTGAAA7250.010.346039
GACGTGA6550.010.2920467
GACGGAG750.002660082710.127767
TCTATAC1606.1281753E-910.0980653
TAGGACG12850.010.0587744
Produced by FastQC (version 0.11.5)