FastQCFastQC Report
Thu 26 May 2016
SRR1511940_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511940_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1458938
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT46750.3204385655867487No Hit
CTGTAGGACGTGGAATATGGCAAGA46140.3162574420571676No Hit
GTCCTAAAGTGTGTATTTCTCATTT44860.30748393694591547No Hit
CTTTAGGACGTGAAATATGGCGAGG38520.2640276694417446No Hit
GTATCAACGCAGAGTACTTTTTTTT22720.1557297157247258No Hit
CTGAAGGACCTGGAATATGGCGAGA21060.14435157628357065No Hit
GTCCTACAGTGTGCATTTCTCATTT19880.13626350125913508No Hit
ATTTAGAAATGTCCACTGTAGGACG17780.12186946943598699No Hit
GTCCTTCAGTGTGCATTTCTCATTT17040.11679728679354436No Hit
TTTCTAAATTTTCCACCTTTTTCAG16860.1155635126372745No Hit
GAATATGGCAAGAAAACTGAAAATC15280.10473371726557262No Hit
CTGTAGGACCTGGAATATGGCGAGA14890.10206053992698799No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG902.25009E-914.7945651
TAGGACC5800.012.9379274
GTCAACG604.0527503E-412.6810572
CCAACGA1001.4446778E-712.34835219
AATGTCC5300.011.8286098
TCCAACG1701.8189894E-1211.73372718
TGGACCG658.0318935E-411.6907475
AAATGTC5650.011.4321027
GTACTAG1354.642061E-911.272051
AGGACCT13650.011.1340455
GGCGAGG10350.011.10479819
GGACGTG25200.011.0064676
AGGACGT25700.010.9771345
TCTATAC1658.0035534E-1110.9379343
CGGTTTC1408.361894E-910.85569313
GGTATCA6600.010.8077181
GGACCTG13350.010.7438846
TTAGGAC15100.010.6939363
ACCTTTT6450.010.60323615
GACGTGA13900.010.5921117
Produced by FastQC (version 0.11.5)