FastQCFastQC Report
Thu 26 May 2016
SRR1511940_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511940_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1458938
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT51890.35566967204912064No Hit
CTGTAGGACGTGGAATATGGCAAGA47100.3228375708906067No Hit
GTCCTAAAGTGTGTATTTCTCATTT46920.32160379673433687No Hit
CTTTAGGACGTGAAATATGGCGAGG42210.28932003964527625No Hit
GTATCAACGCAGAGTACTTTTTTTT27110.18582009653597345No Hit
CTGAAGGACCTGGAATATGGCGAGA23340.15997938226298855No Hit
GTCCTACAGTGTGCATTTCTCATTT22230.15237110829932457No Hit
GTCCTTCAGTGTGCATTTCTCATTT19680.13489264108550192No Hit
ATTTAGAAATGTCCACTGTAGGACG18550.12714728110447462No Hit
TTTCTAAATTTTCCACCTTTTTCAG17260.1183052329845408No Hit
GAATATGGCAAGAAAACTGAAAATC16940.11611185670672777No Hit
TATCAACGCAGAGTACTTTTTTTTT16510.1131645073334165No Hit
CTGTAGGACCTGGAATATGGCGAGA15380.1054191473523892No Hit
GGTATCAACGCAGAGTACTTTTTTT15210.10425391620480103No Hit
GGAATATGGCGAGAAAACTGAAAAT14900.10212908293566964No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTGC400.00529592114.2430868
GGTATCA7050.013.4019321
CCGTGCT500.001504963813.2935479
TCGAACT905.413549E-712.66486119
TAGGACC6550.012.6217154
GGCGAGG11300.012.18852719
CTCGAAC951.041406E-611.99828918
GAAATGT6600.011.5119316
AAATGTC6200.011.4863597
TGTCCAC6400.011.27577710
AGGACGT27550.011.0374515
GGACGTG27300.010.9584736
GCCGGTT1303.2876414E-810.9562211
GTCGAGG1053.4710538E-610.85559619
TTAGGAC16750.010.7222853
ACCTTTT6300.010.70152115
TAGAAAT7200.010.6903744
TGTAGGA25300.010.66692
CACCTTT6200.010.56781314
GACGTGA15300.010.5504337
Produced by FastQC (version 0.11.5)