FastQCFastQC Report
Thu 26 May 2016
SRR1511939_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511939_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1507060
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT24810.16462516422703807No Hit
GTCCTACAGTGGACATTTCTAAATT16610.11021458999641687No Hit
GGTATCAACGCAGAGTACTTTTTTT15500.10284925616763767No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTGC653.3757497E-614.6138038
GGACCGT852.7023816E-713.4103146
GCGTGCG701.091961E-412.2129649
CCGTGCC802.8694025E-511.8737149
GGTATCA6750.011.41050151
CTATTAG1001.908009E-611.41050151
AAGGCGT1104.974154E-711.2260576
ATTACAC1701.0913936E-1111.17526153
GCGCCAC1053.4698805E-610.85596713
GGCGTGC1158.826246E-710.7379688
GTATCAA14050.010.6931151
TGGACCG803.774305E-410.6863435
GTCCTAA4100.010.6683551
TGGCGAG9200.010.53146818
TTAGGAC5450.010.4575823
GTGTTAG1553.605237E-910.4289531
AGGCGTG1303.8452526E-710.2296627
CTACACT2600.010.2296624
TAGGACC3250.010.2296624
GTACTAT1606.0736056E-910.1030481
Produced by FastQC (version 0.11.5)