FastQCFastQC Report
Thu 26 May 2016
SRR1511939_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511939_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1507060
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT26860.17822780778469338No Hit
GTCCTACAGTGGACATTTCTAAATT18240.12103035048372328No Hit
GGTATCAACGCAGAGTACTTTTTTT16440.10908656589651373No Hit
CTGTAGGACGTGGAATATGGCAAGA16200.1074940612848858No Hit
TATCAACGCAGAGTACTTTTTTTTT16000.1061669741085292No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7000.012.5480741
ACCGTGC802.887049E-511.8666028
AATGCCG907.4416166E-611.613687518
TAGGACC3700.011.55968954
GCAATAC752.0643623E-411.40556053
ATTAGAC1104.935973E-711.232753
GGCGAGG4150.011.219357519
GACGTGA4050.011.0168957
GATATAC2100.010.4567281
GTCCTAA5150.010.381691
AGGACGT7550.010.3229145
GGACGTG7550.010.3164036
GTAGGAC8350.010.2445153
TGGCGAG9700.010.18782818
TGCTAGA1502.424531E-810.1382762
ACACCGT856.6013826E-410.0573826
CTATTAG1053.885298E-510.0020881
TAAACTG2002.5465852E-119.9798655
GTCTTAC1159.670644E-69.9625541
ATGCCGG1054.093984E-59.9545919
Produced by FastQC (version 0.11.5)