FastQCFastQC Report
Thu 26 May 2016
SRR1511938_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511938_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1562622
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT32750.2095836357097238No Hit
CTGTAGGACGTGGAATATGGCAAGA29770.19051312473522067No Hit
GTCCTAAAGTGTGTATTTCTCATTT27250.17438638391114422No Hit
GTATCAACGCAGAGTACTTTTTTTT25210.16133140324403472No Hit
CTTTAGGACGTGAAATATGGCGAGG23560.15077222770446083No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGG350.002153583316.304471
TTGCGAG759.686955E-713.93152618
ATTGCGA551.9623205E-413.8163917
CGGTAGG801.9737436E-613.0775441
TCCAACG2300.012.80268918
GGTATCA6100.012.78521
CCAACGA1405.2750693E-1112.21270119
TATTGCG658.0321997E-411.69079116
TTACACC1552.5465852E-1111.6432794
AGTCGTC1251.8348146E-811.3985228
CCGTGCT752.0763285E-411.3985219
AGGCGTG855.329707E-511.1750217
GCCGGTT1803.6379788E-1211.08189611
GCCGTGC600.00588135811.0818968
TCTTATA1553.0559022E-1011.0449652
TAGGACC5400.010.9056444
TTCAACG700.00147715610.8696472
TCGTCAA1252.243396E-710.6386210
GGCGAGG7150.010.62799319
CGGTTTC1701.382432E-1010.6162713
Produced by FastQC (version 0.11.5)