FastQCFastQC Report
Thu 26 May 2016
SRR1511938_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511938_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1562622
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT35370.2263503265665017No Hit
CTGTAGGACGTGGAATATGGCAAGA31990.20472001546119278No Hit
GTATCAACGCAGAGTACTTTTTTTT30270.1937128748987279No Hit
GTCCTAAAGTGTGTATTTCTCATTT29030.18577749449322997No Hit
CTTTAGGACGTGAAATATGGCGAGG25740.1647231384173524No Hit
TATCAACGCAGAGTACTTTTTTTTT17560.11237522574237403No Hit
GGTATCAACGCAGAGTACTTTTTTT16660.10661567544806101No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC456.791265E-414.76946510
CAGTGCG653.3918986E-614.6071629
GCACCGC400.00528313414.2483676
GGTATCA6950.013.3205841
GGCGTGC500.001505924313.292528
CTAGGAC2300.012.395443
TAGGACC5750.012.2301674
CTAATAC802.8566103E-511.87896253
TAGGTAG752.0674095E-411.4038055
TCCAACG2000.011.40051818
TGTAGAA7450.011.352782
CTAGACA1451.209628E-911.1416484
AGGACGT16550.011.13966755
GTCCGGC600.00590033911.07709911
GGCGAGG6950.011.07244719
GTAGAAC7650.010.9317523
GGACGTG16200.010.9061586
GACGTGG9550.010.8368347
GTCCTAC17750.010.8077181
TGTAGGA16700.010.7550862
Produced by FastQC (version 0.11.5)