FastQCFastQC Report
Thu 26 May 2016
SRR1511937_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511937_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1580622
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT26380.16689632309306082No Hit
GGTATCAACGCAGAGTACTTTTTTT16600.10502194705628544No Hit
TATCAACGCAGAGTACTTTTTTTTT16210.1025545639627944No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCTAT1102.6993803E-912.9692311
CCTAGAC1102.752131E-912.952823
GGTATCA7100.012.7256771
CAAGACG604.0966945E-412.664984
CTAGACT1354.7366484E-911.2577594
TTAATAC2000.010.9235453
GTCCTAC4950.010.5675211
GTAGGAC5850.010.554153
GGCGAGG2900.010.48136219
GCCTTAC1303.7807877E-710.2423671
TAATACA2151.8189894E-1210.1614374
GTATCAA14450.010.13604451
TAGGACC1803.947207E-1010.0264434
TTGTACC1252.5734807E-69.8786844
GTCCAAT1356.3410516E-79.863021
GTATAAT2251.8189894E-129.7221191
GGACGTG6550.09.5712446
AGGACGT6850.09.56806855
GTCCTAA4500.09.5107691
GTACTAA1106.7506786E-59.5107691
Produced by FastQC (version 0.11.5)